![]() These cells were evaluated for toxicity using an apoptosis reporter assay and analyzed on the Attune NxT Flow Cytometer. Cell toxicity was measured using the fluorogenic substrate Invitrogen CellEvent Caspase-3/7 Green Detection Reagent, which reacts with the enzymes caspase-3 and caspase-7, which are activated during apoptosis. The ability to add the CellEvent Caspase-3/7 Green Detection Reagent directly to the live cells in the medium without the need to wash, fix, or permeabilize the cells is ideal for this endpoint assay experiment.
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